Research Scientist, Cell Engineering

Poseida Therapeutics, Inc. is seeking an outstanding candidate to join our immuno-oncology team in San Diego, CA. The Cell Engineering Scientist will be a key technology leader in leveraging our gene engineering technologies (piggyBac® DNA Modification System and Cas-CLOVER™) to create cutting edge oncology therapeutics. The successful candidate will have a strong record of accomplishments and work in a collaborative cross-functional team environment, performing experiments, organizing and analyzing results, communicating and presenting findings in team meetings. The candidate will demonstrate excellent oral and written communication skills, work well independently and as part of a team, and be able to work on multiple projects simultaneously. The position is a hands-on, laboratory-based role.

Required Experience and Skills:

  • PhD or equivalent in cellular biology, molecular biology, immunology, or related field
  • Demonstrated expertise in the genetic manipulation of primary human T cells and/or laboratory cell lines using the latest gene editing tools (CRISPRs, TALENs and/or ZFNs)
  • Generation of lines with stable gene integrations (using transposon systems, CRISPRs, viral transduction, and/or other)
  • Molecular biology, Cloning, PCR, NGS
  • Vector design, especially multicistronic DNA vectors
  • Electroporation techniques and technologies (Plasmid DNA, IVT RNA)
  • Multi-parameter flow cytometry and proficiency in FACS data analysis programs
  • Ability to organize, communicate, and present complex data sets to key stakeholders from diverse backgrounds
  • Excellent oral and communication skills

Preferred Experience and Skills:

  • A detailed knowledge of cellular and/or tumor immunology principles, technologies, and experimental techniques (cell culture, in vitro cellular assays, flow cytometry, etc…)
  • Functional assays measuring cellular activation, cytokine release, proliferation, and killing
  • Experience with cell separation technologies (e.g., Miltenyi AutoMACS) and FACS sorting is a plus
  • Demonstrated track record in successfully developing and employing in vitro/in vivo models
  • Strong understanding of transplantation biology and the role of histocompatibility antigens
  • Murine tumor models (genetically engineered or syngeneic)