Description

Poseida Therapeutics, Inc. is seeking an outstanding candidate to join our immuno-oncology team at La Jolla, San Diego, CA.  The Research Associate (RA) will possess a Master’s Degree in the field of immunology, molecular biology, or related field.  Prior hands-on experience developing, testing, and/or utilizing chimeric antigen receptors (CAR)s is highly preferred.  Research studies will support the development of CAR-T cellular therapies against cancer and involve bench work, laboratory studies, and the coordination of research projects with the assistance of a senior lab member and/or the lab director.  Studies may include work with molecular biology and cloning techniques, tissue culture of human cell lines, isolation of cells from blood products, virus production, in vitro human cell based immunoassays, and cell separation techniques.  The candidate will have good oral and written communication skills, and will work with minimal supervision in the laboratory to manage daily research projects, collect data, and prepare reports.

Poseida is currently developing CAR-T and gene therapy products for cancer and orphan diseases.  Poseida is a pre-IPO company with $30M in Series A financing and is currently partnered with J&J’s Janssen to develop allogeneic CAR-T therapies against cancer.  Poseida has entered into a worldwide License Agreement with Janssen to research, develop, manufacture and commercialize licensed products using Janssen Centyrin technology.  Centyrins are non-scFv, alternative binders that are fully human and one fifth the size of a traditional scFv.  In addition, Poseida boasts best-in-class genome engineering capabilities for stable transgene integration or targeted gene disruption.  Proprietary tools include piggyBac™ DNA Modification System (capable of delivering 300kb+ of cargo for stable integration and long-term expression), Footprint-Free™ Gene Editing System, NextGEN™ CRISPR, and XTN™ TALEN, which are synthetic, non-viral, and can be surgically precise.  Poseida aims to advance the first CAR-T product against multiple myeloma and gene therapy product against Progressive Familial Intraheptic Cholestasis 3 into Phase I clinical trials in 2017.  Please visit www.poseida.com for more information.

Qualifications
Required Experience and Skills:

  • MS in the field of immunology, molecular biology, or related field
  • Expertise in the characterization, culture, expansion, and manipulation of T cells
  • A strong knowledge of cellular and/or tumor immunology principles, technologies, and experimental techniques (cell culture, in vitro cellular assays, flow cytometry, etc…)
  • Multi-parameter flow cytometry and proficiency in FACS data analysis programs
  • Functional assays measuring cellular activation, cytokine release, proliferation, and killing
  • Molecular biology, Cloning and PCR technologies, genetic manipulation
  • Cell separation technologies (e.g., Miltenyi AutoMACS) and FACS sorting
  • Ability to organize, communicate, and present complex data sets to key stakeholders from diverse backgrounds

Preferred Experience and Skills:

  • Hands-on experience developing, testing, and/or utilizing CARs
  • Electroporation techniques and technologies (Plasmid DNA, IVT RNA)
  • Demonstrated track record in successfully developing and employing in vitro/in vivo models
  • Genetic manipulation using CRISPRs, TALENs, or ZFNs
  • Viral vector design and transduction techniques (retroviral, lentiviral)
  • Strong understanding of transplantation biology and the role of histocompatibility antigens
  • Murine tumor models (genetically engineered or syngeneic)