Description

Poseida Therapeutics, Inc. is seeking an outstanding candidate to join our immuno-oncology team at La Jolla, San Diego, CA.  The Cell Engineering Research Scientist will have a PhD or equivalent in cellular biology, molecular biology, or related field and ≥ 2 years of experience.  The Scientist will have demonstrated expertise in the genetic manipulation of primary and/or laboratory human cell lines using the latest gene editing tools (CRISPRs, TALENs and/or ZFNs), as well as the generation of lines with stable gene integrations (using viral transduction, transposon system, or other).  Prior experience designing and utilizing multicistronic vectors is a plus.  The Scientist/Associate will plan and perform experiments for the development and evaluation of engineered human cell lines.  The position will play a critical role in the generation and evaluation of engineered cell lines for multiple applications.  The successful candidate will demonstrate excellent oral and written communication skills, work well independently and as part of a team, and be able to work on multiple projects simultaneously.  Broad expertise cellular biology, molecular biology, and flow cytometry is required for this role.  The position is a hands-on, laboratory-based role with the possibility of direct reports.

Poseida is currently developing CAR-T and gene therapy products for cancer and orphan diseases.  Poseida is a pre-IPO company with $30M in Series A financing and is currently partnered with J&J’s Janssen to develop allogeneic CAR-T therapies against cancer.  Poseida has entered into a worldwide License Agreement with Janssen to research, develop, manufacture and commercialize licensed products using Janssen Centyrin technology.  Centyrins are non-scFv, alternative binders that are fully human and one fifth the size of a traditional scFv.  Poseida boasts best-in-class genome engineering capabilities for stable transgene integration or targeted gene disruption.  Proprietary tools include piggyBac™ DNA Modification System (capable of delivering 300kb+ of cargo for stable integration and long-term expression), Footprint-Free™ Gene Editing System, NextGEN™ CRISPR, and XTN™ TALEN, which are synthetic, non-viral, and can be surgically precise.  Poseida aims to advance the first CAR-T product against multiple myeloma and gene therapy product against Progressive Familial Intraheptic Cholestasis 3.  Please visit www.poseida.com for more information.

Qualifications
Required Experience and Skills:

  • PhD or equivalent in cellular biology, molecular biology, or related field and ≥ 2 years of experience in the field
  • Expertise in the characterization, culture, expansion, and manipulation of primary and laboratory cell lines
  • Genetic manipulation using CRISPRs, TALENs, and/or ZFNs
  • Stable gene knock-ins using viral transduction, transposon-based systems, electroporation, or other
  • Molecular biology, Cloning and PCR technologies, genetic manipulation
  • Vector design, especially multicistronic DNA vectors
  • Electroporation techniques and technologies (Plasmid DNA, IVT RNA)
  • Multi-parameter flow cytometry and proficiency in FACS data analysis programs
  • Cell separation technologies (e.g., Miltenyi AutoMACS) and FACS sorting
  • Ability to organize, communicate, and present complex data sets to key stakeholders from diverse backgrounds
  • Excellent oral and communication skills


Preferred Experience and Skills:

  • A detailed knowledge of cellular and/or tumor immunology principles, technologies, and experimental techniques (cell culture, in vitro cellular assays, flow cytometry, etc…)
  • Functional assays measuring cellular activation, cytokine release, proliferation, and killing
  • Demonstrated track record in successfully developing and employing in vitro/in vivo models
  • Strong understanding of transplantation biology and the role of histocompatibility antigens
  • Murine tumor models (genetically engineered or syngeneic)
  • Work with external academic, biotechnology, and contract research organizations
  • Mentor and guide other scientists in the group as needed
  • Previous supervisory skills