Description
Poseida Therapeutics, Inc. is seeking an outstanding candidate to join our immuno-oncology team at La Jolla, San Diego, CA.  The Research Technician (RA) will possess a Bachelor’s degree in the field of immunology, molecular biology, or related field.  Prior hands-on experience cloning and/or tissue culture of cell lines is required.  Research studies will support the development of CAR-T cellular therapies against cancer and involve bench work, laboratory studies, and the coordination of research projects with the assistance of a senior lab member and/or the lab director.  Studies may include work with molecular biology and cloning techniques, tissue culture of human cell lines, isolation of cells from blood products, virus production, in vitro human cell based immunoassays, and cell separation techniques.  The candidate will have good oral and written communication skills, and will work with minimal supervision in the laboratory to manage daily research projects, collect data, and prepare reports.

Poseida Therapeutics is translating best-in-class gene editing technologies into lifesaving treatments. The company is developing CAR T-cell immunotherapies for multiple myeloma and other cancer types, as well as gene therapies for orphan diseases. Poseida has assembled a suite of industry-leading gene editing technologies, including the piggyBac™ DNA Modification System, XTN™ TALEN and NextGEN™ CRISPR site-specific nucleases, and Footprint-Free™ Gene Editing (FFGE).  For more information, visit www.poseida.com.

Qualifications
Required Experience and Skills:

  • BS in the field of immunology, molecular biology, or related field
  • Knowledge of cellular principles, technologies, and experimental techniques (cell culture, etc…)
  • Molecular biology, Cloning and PCR technologies, genetic manipulation
  • Cell separation techniques (e.g., magnetic beads, Miltenyi AutoMACS)
  • Ability to organize, communicate, and present data to key stakeholders from diverse backgrounds

Preferred Experience and Skills:

  • Expertise in the characterization, culture, expansion, and manipulation of T cells
  • Electroporation techniques and technologies (Plasmid DNA, in vitro transcribed mRNA)
  • Functional assays measuring cellular activation, cytokine release, proliferation, and killing
  • Multi-parameter flow cytometry and proficiency in FACS data analysis programs
  • Demonstrated track record in successfully developing and employing in vitro/in vivo models
  • Genetic manipulation using CRISPRs, TALENs, or ZFNs
  • Viral vector design and transduction techniques (retroviral, lentiviral)
  • Expertise in murine tumor models (genetically engineered or syngeneic)